A method to prevent SARS-CoV-2 IgM false positives in gold immunochromatography and enzyme-linked immunosorbent assays.
Identifieur interne : 000A31 ( 2020/Analysis ); précédent : 000A30; suivant : 000A32A method to prevent SARS-CoV-2 IgM false positives in gold immunochromatography and enzyme-linked immunosorbent assays.
Auteurs : Qiang Wang [République populaire de Chine] ; Qin Du [République populaire de Chine] ; Bin Guo [République populaire de Chine] ; Daiyong Mu [République populaire de Chine] ; Xiaolan Lu [République populaire de Chine] ; Qiang Ma [République populaire de Chine] ; Yangliu Guo [République populaire de Chine] ; Li Fang [République populaire de Chine] ; Bing Zhang [République populaire de Chine] ; Guoyuan Zhang ; Xiaolan GuoSource :
- Journal of clinical microbiology [ 1098-660X ] ; 2020.
Abstract
Objective: To investigate the interference factors that lead to false-positive novel coronavirus (SARS-CoV-2) IgM detected using gold immunochromatography assay (GICA) and enzyme-linked immunosorbent assay (ELISA) and the corresponding solutions.Methods: GICA and ELISA were used to detect SARS-CoV-2 IgM in 86 serum samples, including 5 influenza A virus (Flu A) IgM-positive sera, 5 influenza B virus (Flu B) IgM-positive sera, 5 Mycoplasma pneumoniae IgM-positive sera, 5 Legionella pneumophila IgM-positive sera, 6 sera of HIV infection patients, 36 rheumatoid factor IgM (RF-IgM)-positive sera, 5 sera from hypertensive patients, 5 sera from diabetes mellitus patients, and 14 sera from novel coronavirus infection disease (COVID-19) patients. The interference factors causing false-positive reactivity in the two methods were analyzed, and the urea dissociation test was employed to dissociate the SARS-CoV-2 IgM-positive serum using the best dissociation concentration.Results: Two methods detected positive SARS-CoV-2 IgM in 22 middle-high level RF-IgM-positive sera and 14 sera from COVID-19 patients; the other 50 sera were negative. When urea dissociation concentration was 6 mol/L, SARS-CoV-2 IgM were positive in 1 middle-high level RF-IgM-positive sera and in 14 COVID-19 patient sera detected using GICA. When urea dissociation concentration was 4 mol/L and the avidity index (AI) lower than 0.371 was set to negative, SARS-CoV-2 IgM were positive in 3 middle-high level RF-IgM-positive sera and in 14 COVID-19 patient sera detected using ELISA.Conclusion: Middle-high level of RF-IgM could lead to false-positive reactivity of SARS-CoV-2 IgM detected using GICA and ELISA, and urea dissociation tests would be helpful in reducing false-positive results of SARS-CoV-2 IgM.
DOI: 10.1128/JCM.00375-20
PubMed: 32277023
Affiliations:
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<front><div type="abstract" xml:lang="en"><b>Objective:</b>
To investigate the interference factors that lead to false-positive novel coronavirus (SARS-CoV-2) IgM detected using gold immunochromatography assay (GICA) and enzyme-linked immunosorbent assay (ELISA) and the corresponding solutions.<b>Methods:</b>
GICA and ELISA were used to detect SARS-CoV-2 IgM in 86 serum samples, including 5 influenza A virus (Flu A) IgM-positive sera, 5 influenza B virus (Flu B) IgM-positive sera, 5 <i>Mycoplasma pneumoniae</i>
IgM-positive sera, 5 <i>Legionella pneumophila</i>
IgM-positive sera, 6 sera of HIV infection patients, 36 rheumatoid factor IgM (RF-IgM)-positive sera, 5 sera from hypertensive patients, 5 sera from diabetes mellitus patients, and 14 sera from novel coronavirus infection disease (COVID-19) patients. The interference factors causing false-positive reactivity in the two methods were analyzed, and the urea dissociation test was employed to dissociate the SARS-CoV-2 IgM-positive serum using the best dissociation concentration.<b>Results:</b>
Two methods detected positive SARS-CoV-2 IgM in 22 middle-high level RF-IgM-positive sera and 14 sera from COVID-19 patients; the other 50 sera were negative. When urea dissociation concentration was 6 mol/L, SARS-CoV-2 IgM were positive in 1 middle-high level RF-IgM-positive sera and in 14 COVID-19 patient sera detected using GICA. When urea dissociation concentration was 4 mol/L and the avidity index (AI) lower than 0.371 was set to negative, SARS-CoV-2 IgM were positive in 3 middle-high level RF-IgM-positive sera and in 14 COVID-19 patient sera detected using ELISA.<b>Conclusion:</b>
Middle-high level of RF-IgM could lead to false-positive reactivity of SARS-CoV-2 IgM detected using GICA and ELISA, and urea dissociation tests would be helpful in reducing false-positive results of SARS-CoV-2 IgM.</div>
</front>
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<name sortKey="Lu, Xiaolan" sort="Lu, Xiaolan" uniqKey="Lu X" first="Xiaolan" last="Lu">Xiaolan Lu</name>
<name sortKey="Ma, Qiang" sort="Ma, Qiang" uniqKey="Ma Q" first="Qiang" last="Ma">Qiang Ma</name>
<name sortKey="Mu, Daiyong" sort="Mu, Daiyong" uniqKey="Mu D" first="Daiyong" last="Mu">Daiyong Mu</name>
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